| Source : |
HEK293 |
| Species : |
Cynomolgus/Rhesus macaque |
| Tag : |
His-Avi |
| Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
| Bio-activity : |
Immobilized Biotinylated Cynomolgus / Rhesus macaque 4-1BB, Avitag,His Tag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Cynomolgus 4-1BB Ligand, Fc Tag with a linear range of 0.3-2 ng/mL.Immobilized Cynomolgus 4-1BB Ligand, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus / Rhesus macaque 4-1BB, Avitag,His Tag with a linear range of 0.3-20 ng/mL.Immobilized Human 4-1BB Ligand, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus / Rhesus macaque 4-1BB, Avitag,His Tag with a linear range of 0.6-20 ng/mL.Immobilized Utomilumab at 1 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus / Rhesus macaque 4-1BB, Avitag,His Tag with a linear range of 0.3-10 ng/mL. |
| Molecular Mass : |
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.The protein has a calculated MW of 21.8 kDa. The protein migrates as 30-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Protein Length : |
Leu 24-Gln 186 |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>90% as determined by SDS-PAGE.>90% as determined by SEC-MALS. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
