| Source : |
HEK293 |
| Species : |
Cynomolgus/Rhesus macaque |
| Tag : |
His-Avi |
| Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant. |
| Bio-activity : |
Immobilized Human APRIL, His,Flag Tag, active trimer at 2 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus / Rhesus macaque BCMA, His,Avitag with a linear range of 0.2-1 ng/mL.Immobilized Biotinylated Cynomolgus / Rhesus macaque BCMA, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind Human BAFF, Fc Tag, active trimer with a linear range of 0.2-5 ng/mL. |
| Molecular Mass : |
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)The protein has a calculated MW of 9.6 kDa. The protein migrates as 15-18 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Protein Length : |
Met 1-Ala 53 |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>90% as determined by SDS-PAGE. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
