Active Recombinant DENV2 (strain Thailand/16681/1984) Envelope Protein, His-tagged

• Cat.No.: Envelope-11083D
• Product Overview: DENV2 (strain Thailand/16681/1984) Envelope Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Met 281 - Ser 677 (Accession # P29990). This protein carries a polyhistidine tag at the N-terminus. The protein is designed as a dimer.

Specification

• Species: DENV2
• Source: HEK293
• Tag: His
• Protein Length: 281-677 aa
• Description: Dengue is a mosquito-borne viral disease widely spread all over the world transmitted by 4 serotypes of dengue virus (DENV). The symptoms range from mild dengue fever to dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) with high mortality. Currently, most of the studies on DENV vaccines emphasize on its envelope protein which is essential for viral attachment and fusion (i.e., Sanofi’s tetravalent dengue vaccine Dengvaxia). However, the vaccine of envelope protein still needs to be improved to maximize its efficiency on people never infected with DENV before, which requires more research to be conducted on the DENV envelope protein.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, 0.5 M Arginine, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized DENV2 (strain Thailand/16681/1984) Envelope Protein, His Tag at 1 μg/mL (100 μL/well) can bind Human Anti-E(DENV) Antibody EDE2 A11, Human IgG1 | Human Kappa with a linear range of 1-39 ng/mL.
• Molecular Mass: The protein has a calculated MW of 46.2 kDa. The protein migrates as 55-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU/μg by the LAL method/rFC method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Official Symbol: Envelope
• Synonyms: Envelope Protein; E Protein; Genome polyprotein
• UniProt ID: P29990