Active Recombinant DENV2 (strain Thailand/16681/1984) NS1 protein, His-tagged
Cat.No. : | NS1-0576D |
Product Overview : | Recombinant DENV2 (strain Thailand/16681/1984) NS1 protein(Asp776-Ala1127), fused with His tag, was expressed in HEK293. |
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Source : | HEK293 |
Species : | DENV2 (strain Thailand/16681/1984) |
Tag : | His |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant. |
Bio-activity : | Immobilized DENV2 (strain Thailand/16681/1984) NS1 protein, His Tag at 1 μg/mL (100 μL/well) can bind Mouse Anti-NS1(DENV) Antibody 2B7,Mouse IgG1 with a linear range of 0.1-2 ng/mL. |
Molecular Mass : | This protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 41.8 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Proteinlength : | Asp776-Ala1127 |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >90% as determined by SDS-PAGE. >90% as determined by SEC-MALS. |
Storage : | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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