Active Recombinant E.coli Exonuclease I

• Cat.No.: Exonuclease I-25E
• Product Overview: Exonuclease I is derived from a plasmid carrying the E.coli exo I gene and is obtained through recombinant expression and purification. DNA Exonuclease Activity: Negative DNA Endonuclease Activity: Negative RNase Activity: Negative Residual gDNA: ≤1 E.coli genome

Specification

• Species: E.coli
• Tag: Non
• Description: It is a DNA-specific exonuclease that can catalyze the removal of nucleotides from linear single-stranded DNA in the 3' to 5' direction. It can gradually release deoxyribonucleic acid 5' monophosphate, but the terminal dinucleotide will remain intact. Moreover, it has no activity against double-stranded DNA or RNA.
• Purity: ≥ 95% by SDS-PAGE
• Bio-activity: 20U/μL
• Unit definition: One unit refers to the amount of enzyme required to catalyze the release of 10 nmol of acid-soluble nucleotides within 30 minutes at 37 centigrade in a 50 μL reaction system.
• Usage: 1. Reaction system: PCR products: 5 μL Exonuclease I (E.coli): 0.5 μL (10U) 2. Incubate the mixture at 37 centigrade for 15 minutes. 3. Inactivate the Exonuclease I (E.coli) at 80 centigrade for 15 minutes. 4. The Purified product can then be used for downstream applications.
• Applications: 1. Remove single-stranded primers in PCR reactions before Sanger DNA sequencing or SNP analysis. 2. Remove single-stranded primers in nested PCR reactions. 3. Remove single-stranded DNA with 3'-hydroxyl termini from nucleic acid mixtures. 4. Enzymatic purification of PCR products.
• Note: 1. This product should be placed on ice when in use, and it must be immediately returned to the storage at -20 centigrade after use; 2. Try to avoid freeze-thaw cycles of this product after receipt; 3. Please wear lab coat and disposable gloves when using; 4. This product should not be used directly for clinical diagnosis and treatment.
• Stability: This product should be stored at -20 centigrade and can be stored for at least 12 months.
• Storage: Store at -20 centigrade.
• Storage Buffer: 10mM Tris-HCl, 100mM NaCl, 5mM β-ME, 0.5mM EDTA, 100 μg/ml BSA, 50% Glycerol, pH 7.5
• Shipping: Dry Ice