| Species : |
E.coli |
| Tag : |
Non |
| Description : |
It is a DNA-specific exonuclease that can catalyze the removal of nucleotides from linear single-stranded DNA in the 3' to 5' direction. It can gradually release deoxyribonucleic acid 5' monophosphate, but the terminal dinucleotide will remain intact. Moreover, it has no activity against double-stranded DNA or RNA. |
| Purity : |
≥ 95% by SDS-PAGE |
| Bio-activity : |
20U/μL |
| Unit definition : |
One unit refers to the amount of enzyme required to catalyze the release of 10 nmol of acid-soluble nucleotides within 30 minutes at 37 centigrade in a 50 μL reaction system. |
| Usage : |
1. Reaction system:
PCR products: 5 μL
Exonuclease I (E.coli): 0.5 μL (10U)
2. Incubate the mixture at 37 centigrade for 15 minutes.
3. Inactivate the Exonuclease I (E.coli) at 80 centigrade for 15 minutes.
4. The Purified product can then be used for downstream applications. |
| Applications : |
1. Remove single-stranded primers in PCR reactions before Sanger DNA sequencing or SNP analysis.
2. Remove single-stranded primers in nested PCR reactions.
3. Remove single-stranded DNA with 3'-hydroxyl termini from nucleic acid mixtures.
4. Enzymatic purification of PCR products. |
| Note : |
1. This product should be placed on ice when in use, and it must be immediately returned to the storage at -20 centigrade after use;
2. Try to avoid freeze-thaw cycles of this product after receipt;
3. Please wear lab coat and disposable gloves when using;
4. This product should not be used directly for clinical diagnosis and treatment. |
| Stability : |
This product should be stored at -20 centigrade and can be stored for at least 12 months. |
| Storage : |
Store at -20 centigrade. |
| Storage Buffer : |
10mM Tris-HCl, 100mM NaCl, 5mM β-ME, 0.5mM EDTA, 100 μg/ml BSA, 50% Glycerol, pH 7.5 |
| Shipping : |
Dry Ice |
