Active Recombinant Full Length Human BUB1 Protein, GST-tagged

• Cat.No.: BUB1-5059HF
• Product Overview: Full-length human BUB1 [1-1085 (end) amino acids of accession number NP_004327] was co-expressed as N-terminal GST-fusion protein (149 kDa) with DYKDDDDK tagged BUB3 [1-328 (end) amino acids of accession number NP_004716] using baculovirus expression system. GST-BUB1 was purified by using glutathione sepharose chromatography.

Specification

• Source: Insect (sf21)
• Species: Human
• Tag: GST
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device.
• Protein length: 1-1085(end)
• Purity: 70%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: BUB1 mitotic checkpoint serine/threonine kinase
• Official Symbol: BUB1
• Synonyms: BUB1;BUB1A;BUB1L;hBUB1
• Protein Refseq: NP_004327
• UniProt ID: O43683