Active Recombinant Full Length Human CDK4 Protein, GST-tagged

• Cat.No.: CDK4-4867HF
• Product Overview: Full-length human CDK4 [1-303(end) amino acids of accession number NP_000066.1] was co-expressed as N-terminal GST-fusion protein (61 kDa) with human GST-CyclinD3 [1-292(end) amino acids of accession number AAA51927.1] using baculovirus expression system. GST-CDK4/CycD3 was purified by using glutathione sepharose chromatography and activated with His-tagged CDK7. Activated GST- CDK4/CycD3 was purified using glutathione sepharose chromatography.

Specification

• Source: Insect (sf21)
• Species: Human
• Tag: GST
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
• Protein length: 1-303(end)
• Purity: 84%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: cyclin-dependent kinase 4
• Official Symbol: CDK4
• Synonyms: CDK4;CMM3;PSK-J3
• Protein Refseq: NP_000066.1
• UniProt ID: P11802