Active Recombinant Full Length Human LIMK2 Protein, GST-tagged

• Cat.No.: LIMK2-4132HF
• Product Overview: Full-length human LIMK2 [1-638(end) amino acids of accession number NP_005560.1] was co-expressed as N-terminal GST-fusion protein (99 kDa) with human His-tagged ROCK2 [1-553 amino acids of accession number NP_004841.2] using baculovirus expression system. GST-LIMK2 was purified by using glutathione sepharose chromatography.

Specification

• Species: Human
• Source: Sf21 Cells
• Tag: GST
• Protein Length: 1-638
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione- coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG(or HRP-labeled anti-mouse IgG) .
• Purity: 70%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: LIM domain kinase 2
• Official Symbol: LIMK2
• Synonyms: LIMK2
• Protein Refseq: NP_005560.1
• UniProt ID: P53671