Active Recombinant Full Length Human MAPKAPK3 Protein, C-Flag-tagged

• Cat.No.: MAPKAPK3-523HFL
• Product Overview: Recombinant Full Length Human MAPKAPK3 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation. Alternate splicing results in multiple transcript variants that encode the same protein.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: MAPKAPK3 activity verified in a biochemical assay: MAPKAPK3 (mitogen-activated protein kinase-activated protein kinase 3) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. MAPKAPK3 is a serine/threonine kinase that functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. Varying concentrations of MAPKAPK3 were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “ΔR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors.
• Molecular Mass: 42.8 kDa
• AA Sequence: MDGETAEEQGGPVPPPVAPGGPGLG GAPGGRREPKKYAVTDDYQLSKQVL GLGVNGKVLECFHRRTGQKC ALKLLYDSPKARQEVDHHWQASGGP HIVCILDVYENMHHGKRCLLIIMEC MEGGELFSRIQERGDQAFTE REAAEIMRDIGTAIQFLHSHNIAHR DVKPENLLYTSKEKDAVLKLTDFGF AKETTQNALQTPCYTPYYVA PEVLGPEKYDKSCDMWSLGVIMYIL LCGFPPFYSNTGQAISPGMKRRIRL GQYGFPNPEWSEVSEDAKQL IRLLLKTDPTERLTITQFMNHPWIN QSMVVPQTPLHTARVLQEDKDHWDE VKEEMTSALATMRVDYDQVK
  IKDLKTSNNRLLNKRRKKQAGSSSA SQGCNNQTRTRPLEQKLISEEDLAA NDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: MAPK signaling pathway, VEGF signaling pathway

Gene Information

• Gene Name: MAPKAPK3 MAPK activated protein kinase 3 [ Homo sapiens (human) ]
• Official Symbol: MAPKAPK3
• Synonyms: 3PK; MK3; MK-3; MDPT3; MAPKAP3; MAPKAP-K3; MAPKAPK-3
• Gene ID: 7867
• mRNA Refseq: NM_004635.5
• Protein Refseq: NP_004626.1
• MIM: 602130
• UniProt ID: Q16644