Active Recombinant Full Length Human MAPKAPK3 Protein, GST-tagged

• Cat.No.: MAPKAPK3-3301HF
• Product Overview: Full-length human MAPKAPK3 [1-382(end) amino acids of accession number NP_004626.1] was co- expressed as N-terminal GST-fusion protein (70 kDa) with human His-tagged p38β [1-364(end) amino acids of accession number NP_002742.3] and human His-tagged MAP2K6 [1-334(end) amino acids of accession number NP_002749.2] using baculovirus expression system. GST-MAPKAPK3 was purified by using glutathione sepharose chromatography and Ni-NTA chromatography.

Specification

• Species: Human
• Source: Sf21 Cells
• Tag: GST
• Protein Length: 1-382
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
• Purity: 76%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: mitogen-activated protein kinase-activated protein kinase 3
• Official Symbol: MAPKAPK3
• Synonyms: MAPKAPK3;3PK;MAPKAP-K3;MAPKAP3;MAPKAPK-3;MDPT3;MK-3;MK3;3pK
• Protein Refseq: NP_004626.1
• UniProt ID: Q16644