Active Recombinant Full Length Human PPP2CA and PPP2R1A complex, N-His tagged

• Cat.No.: PPP2CA-PPP2R1A-10HFL
• Product Overview: Recombinant full length human PP2Aa and human PPP2R1A were expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. The PP2Aa gene accession number is NM_002715; PPP2R1A is NM_014225.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Protein Length: Full length
• Description: PP2A alpha is also known as Protein phosphatase 2A active subunit. PP2A is one of the four major Ser/Thr phosphatases and is a human tumor suppressor that inhibits cellular transformation. PP2A alpha gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase-2A. Protein phosphatase 2A holoenzyme is a heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. PTP2A alpha can alter the substrate specificity of PP2A leading to enhanced phosphotyrosine phosphatase activity and decreased phosphoserine phosphatase activity.
• Bio-activity: 4,000 nmol/min/mg
• Molecular Mass: PP2Aalpha: ~38 kDa
  PPP2R1A: ~65 kDa
• Purity: >75%
• Stability: 1 year at –70 centigrade from date of shipment
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μL
• Storage Buffer: Recombinant protein stored in 50mM MOPS, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
• Shipping: Dry ice
• References: 1. Van Hoof, C. et.al: Structure and chromosomal localization of the human gene of the phosphotyrosyl phosphatase activator (PTPA) of protein phosphatase 2A. Genomics 28: 261-272, 1995.
  2. Chao, Y. et.al: Structure and mechanism of the phosphotyrosyl phosphatase activator. Molec. Cell 23: 535-546, 2006.

Gene Information

• Gene Name: PPP2CA protein phosphatase 2 catalytic subunit alpha [ Homo sapiens (human) ]
• Official Symbol: PPP2CA
• Synonyms: PPP2CA; protein phosphatase 2 catalytic subunit alpha; HJS3; RP-C; PP2Ac; PP2CA; NEDLBA; PP2Calpha; serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; PP2A-alpha; protein phosphatase 2, catalytic subunit, alpha isozyme; replication protein C; EC 3.1.3.16; serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform; Names; medium tumor antigen-associated 61 KDA protein; protein phosphatase 2, regulatory subunit A, alpha; testicular secretory protein Li 1; EC 3.1.3.16; PPP2R1A; protein phosphatase 2 scaffold subunit Aalpha; HJS2; MRD36; PP2AA; PR65A; PP2AAALPHA; PP2A-Aalpha
• Gene ID: 5515
• mRNA Refseq: NM_002715
• Protein Refseq: NP_002706
• MIM: 176915
• UniProt ID: P05323