Active Recombinant Full Length Human SNCA Pre-formed Fibrils Protein, His Tagged

• Cat.No.: SNCA-11260H
• Product Overview: Human Alpha-Synuclein Pre-formed Fibrils, His Tag is expressed from E.coli cells. It contains AA Met 1-Ala 140 (Accession # P37840-1). This protein carries a polyhistidine tag at the C-terminus. Predicted N-terminus: Met 1

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-140 aa
• Description: Alpha-synuclein is a neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. It acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DJC5. Abnormalities in alpha-synuclein are implicated in the pathogenesis of Parkinson's disease (PD). Alpha-synuclein is present in Lewy-bodies, the neuropathological hallmark of PD, and the protein and its aggregation have been widely linked to neurotoxic pathways that ultimately lead to neurodegeneration.
• Form: Supplied as 0.2 μm filtered solution in PBS, pH7.4.
• Bio-activity: Thioflavin T is a sensitive fluorescence reporter of fibrils formulation that binds to beta sheet-rich structures. The alpha synuclein pre-formed fibrils is able to induce the aggregation of alpha synuclein monomers.
• Molecular Mass: The protein has a calculated MW of 16.3 kDa.
• Endotoxin: Less than 1.0 EU/μg by the LAL method/rFC method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: This product is stable after storage at: The product should be stored at -70 centigrade or room temperature for short storage. Do not store fibrils on ice or at 4 centigrade; The unsonicated fibril is validated to be stable after storage at -70 centigrade for 1 year under sterile conditions; The sonicated fibril should be stored at -70 centigrade for not more than 8 weeks. Please avoid repeated freeze-thaw cycles.
• Concentration: 0.2 mg/mL
• Shipping: This product is supplied and shipped with dry ice, please inquire the shipping cost.

Gene Information

• Gene Name: SNCA synuclein, alpha (non A4 component of amyloid precursor) [ Homo sapiens (human) ]
• Official Symbol: SNCA
• Synonyms: SNCA; synuclein, alpha (non A4 component of amyloid precursor); PARK1, PARK4, Parkinson disease (autosomal dominant, Lewy body) 4; alpha-synuclein; alpha synuclein; NACP; PD1; synuclein alpha-140; non A-beta component of AD amyloid; PARK1; PARK4; MGC110988
• Gene ID: 6622
• mRNA Refseq: NM_000345
• Protein Refseq: NP_000336
• MIM: 163890
• UniProt ID: P37840