Active Recombinant Full Length Mouse Anpep Protein, His-tagged

• Cat.No.: Anpep-05HFL
• Product Overview: Recombinant mouse Anpep, fused to His-tag at C terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Species: Mouse
• Source: Insect Cells
• Tag: His
• Protein Length: 1-966 aa
• Description: Enables metallopeptidase activity. Involved in angiotensin maturation. Is active in external side of plasma membrane. Is expressed in several structures, including alimentary system; brain; genitourinary system; liver; and sensory organ. Orthologous to human ANPEP (alanyl aminopeptidase, membrane).
• Form: Liquid
• Molecular Mass: 107.5 kDa (943aa)
• Bio-activity: Specific activity is > 4,000 pmol/min/μg, and is defined as the amount of enzyme that hydrolyze 1pmole of H-Ala-AMC to Alanine and AMC per minute at pH7.5 at 25 centigrade.
• Application: SDS-PAGE, Enzyme Activity
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 95% by SDS-PAGE
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
• Concentration: 0.5 mg/mL (determined by absorbance at 280nm)
• Reference: 1. Azimi A., et al. (2017) Cell Death Dis. 8:e3029.

Gene Information

• Gene Name: Anpep alanyl aminopeptidase, membrane [ Mus musculus (house mouse) ]
• Official Symbol: Anpep
• Synonyms: Anpep; alanyl aminopeptidase, membrane; Apn; AP-M; AP-N; Cd13; P150; aminopeptidase N; alanyl (membrane) aminopeptidase; alanyl aminopeptidase; aminopeptidase M; aminopeptidase N/CD13; membrane protein p161; microsomal aminopeptidase; EC 3.4.11.2
• Gene ID: 16790
• mRNA Refseq: NM_008486
• Protein Refseq: NP_032512
• UniProt ID: P97449