Active Recombinant H1N1 (A/California/04/2009) HA0 Protein

• Cat.No.: HA-285V
• Product Overview: Active Recombinant H1N1 (A/California/04/2009) HA0 Protein(Met1-Ile566), termed as HA, was expressed in Insect Cells.

Specification

• Species: H1N1
• Source: Insect Cells
• Tag: Non
• Protein Length: 1-566 aa
• Form: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.5.
• Bio-activity: Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 2-10 μg/mL for 1%GRBC.
• Molecular Mass: The recombinant hemagglutinin of Influenza A virus (A/California/04/2009 (H1N1)) consists 549 amino acids and predicts a molecular mass of 61.5 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.