Active Recombinant H1N1 (A/California/07/2009) HA Protein, His-tagged

• Cat.No.: HA-297V
• Product Overview: Active Recombinant H1N1 (A/California/07/2009) HA Protein(ACP41953.1) (Met1-Gln529), termed as HA, was expressed in Insect Cells with a polyhistidine tag at the C-terminus.

Specification

• Species: H1N1
• Source: Insect Cells
• Tag: His
• Protein Length: 1-529 aa
• Form: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.0.
• Bio-activity: 1. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System. 2. Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 1-6 μg/mL for 1% GRBC.
• Molecular Mass: The recombinant hemagglutinin of Influenza A virus (A/California/07/2009(H1N1))] consists 523 amino acids and predicts a molecular mass of 59 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.