Active Recombinant H1N1 (A/England/195/2009) HA Protein, His-tagged
Cat.No. : | HA-791V |
Product Overview : | Recombinant Influenza A virus (A/England/195/2009 (H1N1)) hemagglutinin (ACR15621.1) (Met1-Gln529), termed as HA, was expressed with a polyhistidine tag at the C-terminus. |
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Species : | H1N1 |
Source : | Insect Cells |
Tag : | His |
ProteinLength : | 1-529 a.a. |
Predicted N Terminal : | Asp 18 |
Form : | Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.0, 5% ~ 8% trehalose and mannitol. |
Bio-activity : | Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 1-4μg/mL for 1% GRBC. |
Molecular Mass : | The recombinant virus HA consists 523 amino acids and predicts a molecular mass of 58.9 kDa. |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method. |
Purity : | >95 % as determined by SDS-PAGE |
Stability : | Samples are stable for up to twelve months from date of receipt at -70ºC. |
Storage : | Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
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Not For Human Consumption!
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