Active Recombinant H1N1 (A/New York/1/1918) HA Protein, His-tagged

• Cat.No.: HA-607V
• Product Overview: Recombinant Influenza A virus (A/New York/1/1918(H1N1)) hemagglutinin (AAD17219.1) (Met1-Gln529) was expressed with a polyhistidine tag at the C-terminus.

Specification

• Source: Baculovirus-Insect Cells
• Species: H1N1
• Tag: His
• Predicted N Terminal: Asp
• Form: Lyophilized from sterile 20 mM Tris, 500 mM NaCl,10 % glycerol, pH 8.0, 5%~8% trehalose and mannitol.
• Bio-activity: 1. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System. 2. Measured by its ability to bind with Neu5Aca2-6GalNAca-PAA-biotin (01-059) using the Octet RED System. 3. Measured by its ability to agglut
• Molecular Mass: The recombinant hemagglutinin of Influenza A virus (A/New York/1/1918(H1N1)) consists 523 amino acids and predicts a molecular mass of 58.6 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Protein length: Met1-Gln529

Reviews

06/01/2021

The transport process does not affect the protein quality, and the stability is good.

11/27/2019

Higher solubility makes it easy to prepare the desired concentration.

Q&As

How about the structural characteristics of HA protein?

HA protein is a glycoprotein that contains thousands of carbohydrates and an amino acid chain consisting of 566 amino acid residues. The structure of HA protein has triple symmetry, consisting of three monomers that form a spiral.

Will genetic variants in HA proteins affect vaccine development?

Genetic variants in the HA protein may affect the development and production of influenza vaccines. Because HA protein is an important component of influenza viruses, different subtypes or variants of HA protein may require different vaccines to prepare.

Why is hemagglutinin protein related to viral replication?

This protein is one of the key factors in the replication process of influenza viruses. When a virus infects a host cell, the receptor-binding region of the HA protein binds to the receptor on the cell surface, allowing the virus to invade the host cell and replicate.

What are the subtypes of HA protein?

There are currently 18 known HA subtypes of influenza viruses, H1-H18. Among them, the H1, H2, H3, H5, H7, and H9 subtypes are associated with human viruses, while other subtypes are generally associated with animal viruses.

What are the physiological functions of HA protein?

The main physiological function of HA protein is to bind to host cells and mediate the entry of influenza viruses into host cells. It contains specific regions that bind glycoprotein receptors that allow the influenza virus to adhere to them. This is an important link that allows the virus to stably bind and enter the cell.

What laboratory techniques are HA proteins used in?

HA protein is widely used in viral research and drug development. Researchers can use HA proteins to test drugs such as influenza virus activators, antibodies and vaccines, and HA proteins are also widely used to develop novel immunoassays and treatments.