Active Recombinant H3N2 (A/California/7/2004) HA Protein, His-tagged

• Cat.No.: HA-641V
• Product Overview: Recombinant Influenza A virus (A/California/7/2004(H3N2)) hemagglutinin (ABW80975.1) (Met1-Trp530) was expressed with a C-terminal polyhistidine tag.

Specification

• Species: H3N2
• Source: Insect Cells
• Tag: His
• Protein Length: 1-530 a.a.
• Predicted N Terminal: Gln 17
• Form: Lyophilized from sterile 20 mM Tris, 500 mM NaCl,10 % glycerol, pH 7.0, 5%~8% trehalose and mannitol.
• Bio-activity: 1. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System. 2. Measured by its ability to bind with Neu5Aca2-6GalNAca-PAA-biotin (01-059) using the Octet RED System. 3. Measured by its ability to agglut
• Molecular Mass: The recombinant hemagglutinin of Influenza A virus (A/California/7/2004(H3N2)) comprises 525 amino acids and has a predicted molecular mass of 59.2 kDa. The apparent molecular mass of the protein is approximately 70.6 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.