Active Recombinant H5N1 (A/Cambodia/S1211394/2008) HA Protein, His-tagged

• Cat.No.: HA-568V
• Product Overview: Recombinant N-terminal segment (Met 1-Gly 341) of the influenza hemagglutinin (A/Cambodia/S1211394/2008 (H5N1)) (ADM95445.1), termed as HA, was fused with a polyhistidine tag at the C-terminus.

Specification

• Species: H5N1
• Source: HEK293
• Tag: His
• Protein Length: 1-341 a.a.
• Predicted N Terminal: Asp 17
• Form: Lyophilized from sterile PBS-2, pH 7.4, 10% gly1, 5%~8% trehalose and mannitol.
• Bio-activity: Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 4-16 μg/mL for 1% GRBC.
• Molecular Mass: The secreted recombinant HA1 subunit of influenza A H5N1 HA (A/Cambodia/S1211394/2008(H5N1)) comprises 526 amino acids and has a predicted molecular mass of 60 kDa. As a result of glycosylation, it migrates as an approximately 83 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.