Active Recombinant H5N1 (A/chicken/India/NIV33487/06) HA Protein, His-tagged

• Cat.No.: HA-393V
• Product Overview: Active Recombinant H5N1 (A/chicken/India/NIV33487/06) HA Protein(Met 1-Gln 531)(ABQ45850.1) (HA1+HA2, uncleaved, cleavage site mutated (RRRKKR-TETR)) was expressed in HEK293, fused with a C-terminal polyhistidine tag.

Specification

• Species: H5N1
• Source: HEK293
• Tag: His
• Protein Length: 1-531 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its ability to agglutinate human red blood cells. HA titer is 10-50 μg/mL for 1% HRBC.
• Molecular Mass: The secreted recombinant influenza A H5N1 HA (A/chicken/India/NIV33487/2006 (H5N1)) comprises 524 amino acids and has a predicted molecular mass of 60 kDa. As a result of glycosylation, it migrates as an approximately 65-75 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 94 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.