Active Recombinant H5N1 (A/Anhui/1/2005) NA protein(H255Y mutation)

• Cat.No.: NA-488H
• Product Overview: Active Recombinant H5N1 (A/Anhui/1/2005) NA protein(ABU94738.1) (Met 1-Lys 449) was expressed in HEK293, the cell lysates are collected, and bio-activity was tested. There is an amino acid change from Histidine to Tyrosine (H255Y mutation) in NA / Neuraminidase.

Specification

• Species: H5N1
• Source: HEK293
• Tag: Non
• Protein Length: 1-449 aa
• Form: Lyophilized from sterile PBS, 0.6% Triton X-100, 7% Trehalose, 6% Mannitol, pH 7.4.
• Bio-activity: Measured by its ability to cleave a fluorogenic substrate, 2'-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid. The specific activity is > 60 U. The specific activity is > 300 U. One unit is defined as the amount of enzyme required to cleave 1 nmole of 2'-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid per minute at pH 7.5 at 37°C.
• Molecular Mass: The recombinant influenza A H5N1 Neuraminidase comprises 450 amino acids and has a predicted molecular mass of 49.2 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publications:
  • The Flexible, Versatile and Fast GreGT Platform of Fully Deleted Helper-Virus Independent Adenoviral Vectors (2024)