Species : |
H7N9 |
Source : |
Insect Cells |
Tag : |
His |
Protein Length : |
1-524 a.a. |
Predicted N Terminal : |
Asp 19 |
Form : |
Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4, 5%~8% trehalose and mannitol. |
Bio-activity : |
1. Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 0.1-0.6 μg/mL for 1% GRBC. 2. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System. 3. Measured by its ability to bin |
Molecular Mass : |
The recombinant hemagglutinin of Influenza A virus (A/Pigeon/Shanghai/S1069/2013(A/H7N9)) comprises 517 amino acids and has a predicted molecular mass of 57.7 kDa. The apparent molecular mass of the protein is approximately 58 kDa in SDS-PAGE under reducing conditions. |
Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method. |
Purity : |
>95 % as determined by SDS-PAGE. |
Stability : |
Samples are stable for up to twelve months from date of receipt at -70ºC. |
Storage : |
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |