Active Recombinant H9N2 (A/Guinea fowl/Hong Kong/WF10/99) HA Protein, His-tagged

• Cat.No.: HA-410V
• Product Overview: Active Recombinant H9N2 (A/Guinea fowl/Hong Kong/WF10/99) HA Protein(AAO46082.1)(Met1-Lys523) was expressed in Insect Cells with a polyhistidine tag at the C-terminus.

Specification

• Species: H9N2
• Source: Insect Cells
• Tag: His
• Protein Length: 1-523 aa
• Form: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.0.
• Bio-activity: Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 0.02-0.1μg/mL for 1% GRBC.
• Molecular Mass: The recombinant hemagglutinin of Influenza A virus (A/Guinea fowl/Hong Kong/WF10/99(H9N2)) consists 516 amino acids and predicts a molecular mass of 58.2 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.