Active Recombinant HIV gp160 Protein, Fc-tagged

• Cat.No.: gp160-330V
• Product Overview: Recombinant HIV-1 envelope glycoprotein gp160 extracellular domain (Ala 29-Ser 661), termed as gp140, was fused with the Fc region of human IgG1 at the C-terminus and a signal peptide at the N-terminus.

Specification

• Species: HIV
• Source: HEK293
• Tag: Fc
• Protein Length: 29-661 a.a.
• Predicted N Terminal: Ala 29
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: Using the Octet RED System, the affinity constant (Kd) of gp140-Fc bound to human CD4 is 0.3 μM.
• Molecular Mass: The recombinant HIV-1 gp140 consists of 874 amino acids after removal of the signal peptide and has a predicted molecular mass of 98 kDa. The apparent molecular mass of the recombinant protein is approximately 210-230 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.