Active Recombinant Human ARSA protein, His tagged

• Cat.No.: ARSA-2324H
• Product Overview: Active Recombinant Human ARSA protein(NP_000478.2) (Met 1-Ala 507) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-507 aa
• Form: Lyophilized from sterile 25mM Tris, 0.15mM NaCl, pH 7.5.
• Bio-activity: Measured by its ability to hydrolyze the substrate 4-Nitrocatechol Sulfate (PNCS). The specific activity is >50 pmoles/min/μg.
• Molecular Mass: The recombinant human ARSA consists of 500 amino acids and predicts a molecular mass of 53 kDa. as estimated by SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ARSA arylsulfatase A [ Homo sapiens ]
• Official Symbol: ARSA
• Synonyms: ARSA; arylsulfatase A; metachromatic leucodystrophy; ASA; cerebroside-sulfatase; MLD
• Gene ID: 410
• mRNA Refseq: NM_000487
• Protein Refseq: NP_000478
• MIM: 607574
• UniProt ID: P15289