Active Recombinant Human ARSA, His tagged

• Cat.No.: ARSA-2324H
• Product Overview: Recombinant Human ARSA (NP_000478.2) (Met 1-Ala 507), fused with a C-terminal polyhistidine tag, was produced in Human Cell.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met 1-Ala 507
• Form: Lyophilized from sterile 50 mM Tris, pH 8.0.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to hydrolyze the substrate 4-Nitrocatechol Sulfate (PNCS) . The specific activity is >50 pmoles/min/μg.
• Molecular Mass: The recombinant human ARSA consists of 500 amino acids and predicts a molecular mass of 53.4 kDa as estimated by SDS-PAGE under reducing conditions. It migrates as an approximately 56.7 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: ARSA arylsulfatase A [ Homo sapiens ]
• Official Symbol: ARSA
• Synonyms: ARSA; arylsulfatase A; metachromatic leucodystrophy; ASA; cerebroside-sulfatase; MLD
• Gene ID: 410
• mRNA Refseq: NM_000487
• Protein Refseq: NP_000478
• MIM: 607574
• UniProt ID: P15289