Active Recombinant Human BAP1 Protein, His-tagged

• Cat.No.: BAP1-554H
• Product Overview: Recombinant Human BAP1 (Accession # NP_004647), fused with an N-terminal 6-His tag, was produced in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Form: X mg/ml (X µM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 1 mM TCEP
• Bio-activity: Recombinant Human His6-BAP1 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-BAP1 concentration of 1-5 µM.
• Molecular Mass: Recombinant Human BAP1, His-tagged has a calculated MW of 82 kDa.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: BAP1 BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) [ Homo sapiens ]
• Official Symbol: BAP1
• Synonyms: BAP1; BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase); ubiquitin carboxyl-terminal hydrolase BAP1; hucep 6; KIAA0272; UCHL2; cerebral protein 6; cerebral protein-13; TPDS; hucep-6; HUCEP-13; FLJ35406; FLJ37180; DKFZp686N04275
• Gene ID: 8314
• mRNA Refseq: NM_004656
• Protein Refseq: NP_004647
• MIM: 603089
• UniProt ID: Q92560
• Chromosome Location: 3p21.31-p21.2