Active Recombinant Human C1GalT1 (Asp45-Pro363) and C1GalT1C1 (Ile28-Asp318) Protein, N-6×His and HA tagged
Cat.No. : | C1GalT1-01H |
Product Overview : | Recombinant Human C1GalT1 (Asp45-Pro363) with N-6×His tag and C1GalT1C1 (Ile28-Asp318) with N-HA fusion protein was expressed in Chinese Hamster Ovary cell line. |
- Specification
- Gene Information
- Related Products
Description : | O-glycosylation is a ubiquitous post-translational modification of secreted and membrane bound proteins. The synthesis of mucin-type O-glycans is initiated by the addition of GalNAc to threonine or serine residues on proteins by polypeptide N-acetylgalactosaminyltransferases (GALNTs). The GalNAc alpha 1-O-Ser/Thr structure is then extended by other glycosyltransferases to form eight types of core O-glycans. Core 1 beta -3-galactosyltransferase (C1GalT1), in particular, synthesizes Gal-beta 1-3GalNAc alpha 1-O-Ser/Thr, a precursor for all core 1 and core 2 based mucin-type O-glycans. These glycans play central roles in many processes, such as angiogenesis, thrombopoiesis, and kidney homeostasis. C1GalT1 forms a stable, non-covalent complex with Cosmc chaperone, C1GalT1C1, which is required for the full activity of C1GalT1. Defective C1GalT1 causes a rare autoimmune disease called Tn syndrome as well as susceptibility to IgA nephropathy. The recombinant C1GalT1 is co-purified with C1GalT1C1. The enzymatic activity of recombinant human C1GalT1 was determined using a phosphatase-coupled assay. |
Source : | CHO |
Species : | Human |
Tag : | N-6×His and HA |
Bio-activity : | Measured by its ability to transfer galactose from UDP-galactose to 4-nitrophenyl-alpha -D-galactosaminide. The specific activity is > 2,750 pmol/min/μg, as measured under the described conditions. |
Molecular Mass : | Predicted Molecular Mass: 38 kDa (C1GalT1) & 35 kDa (C1GalT1C1) SDS-PAGE: 25-42 kDa, reducing conditions |
N-terminal Sequence Analysis : | His (C1GalT1) & Tyr (C1GalT1C1) |
Endotoxin : | < 1.0 EU/μg of the protein by the LAL method. |
Purity : | > 90 %, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 centigrade as supplied. 3 months, -20 to -70 centigrade under sterile conditions after opening. |
Storage Buffer : | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
Shipping : | The product is shipped with polar packs. |
Gene Name : | C1GALT1 core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1 [ Homo sapiens (human) ] |
Official Symbol : | C1GALT1 |
Synonyms : | C1GALT1; core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1; C1GALT; T-synthase; glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1; B3Gal-T8; core 1 O-glycan T-synthase; core 1 UDP-galactose:N-acetylgalactosamine-alpha-R beta 1,3-galactosyltransferase 1; core 1 beta3-Gal-T1; epididymis secretory sperm binding protein; EC 2.4.1.122; B3Gal-T8; Beta-1,3-galactosyltransferase; C1GALT; C1GalT1; Core 1 beta1,3-galactosyltransferase 1; Core 1 Beta3-Gal-T1; Core 1 O-Glycan T-Synthase; core 1 synthase, glycoprotein-N-acetylgalactosamine3-beta-galactosyltransferase, 1; Core 1 UDP-galactose:N-acetylgalactosamine-alpha-R beta1,3-galactosyltransferase 1; EC 2.4.1.122; glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1; T-synthase |
Gene ID : | 56913 |
mRNA Refseq : | NM_020156 |
Protein Refseq : | NP_064541 |
MIM : | 610555 |
UniProt ID : | Q96QH4 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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