Active Recombinant Human C1GalT1 (Asp45-Pro363) and C1GalT1C1 (Ile28-Asp318) Protein, N-6×His and HA tagged

• Cat.No.: C1GalT1-01H
• Product Overview: Recombinant Human C1GalT1 (Asp45-Pro363) with N-6×His tag and C1GalT1C1 (Ile28-Asp318) with N-HA fusion protein was expressed in Chinese Hamster Ovary cell line.

Specification

• Species: Human
• Source: CHO
• Tag: HA&His
• Description: O-glycosylation is a ubiquitous post-translational modification of secreted and membrane bound proteins. The synthesis of mucin-type O-glycans is initiated by the addition of GalNAc to threonine or serine residues on proteins by polypeptide N-acetylgalactosaminyltransferases (GALNTs). The GalNAc alpha 1-O-Ser/Thr structure is then extended by other glycosyltransferases to form eight types of core O-glycans. Core 1 beta -3-galactosyltransferase (C1GalT1), in particular, synthesizes Gal-beta 1-3GalNAc alpha 1-O-Ser/Thr, a precursor for all core 1 and core 2 based mucin-type O-glycans. These glycans play central roles in many processes, such as angiogenesis, thrombopoiesis, and kidney homeostasis. C1GalT1 forms a stable, non-covalent complex with Cosmc chaperone, C1GalT1C1, which is required for the full activity of C1GalT1. Defective C1GalT1 causes a rare autoimmune disease called Tn syndrome as well as susceptibility to IgA nephropathy. The recombinant C1GalT1 is co-purified with C1GalT1C1. The enzymatic activity of recombinant human C1GalT1 was determined using a phosphatase-coupled assay.
• Bio-activity: Measured by its ability to transfer galactose from UDP-galactose to 4-nitrophenyl-alpha -D-galactosaminide. The specific activity is > 2,750 pmol/min/μg, as measured under the described conditions.
• Molecular Mass: Predicted Molecular Mass: 38 kDa (C1GalT1) & 35 kDa (C1GalT1C1)
  SDS-PAGE: 25-42 kDa, reducing conditions
• N-terminal Sequence Analysis: His (C1GalT1) & Tyr (C1GalT1C1)
• Endotoxin: < 1.0 EU/μg of the protein by the LAL method.
• Purity: > 90 %, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  3 months, -20 to -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
• Shipping: The product is shipped with polar packs.

Gene Information

• Gene Name: C1GALT1 core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1 [ Homo sapiens (human) ]
• Official Symbol: C1GALT1
• Synonyms: C1GALT1; core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1; C1GALT; T-synthase; glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1; B3Gal-T8; core 1 O-glycan T-synthase; core 1 UDP-galactose:N-acetylgalactosamine-alpha-R beta 1,3-galactosyltransferase 1; core 1 beta3-Gal-T1; epididymis secretory sperm binding protein; EC 2.4.1.122; B3Gal-T8; Beta-1,3-galactosyltransferase; C1GALT; C1GalT1; Core 1 beta1,3-galactosyltransferase 1; Core 1 Beta3-Gal-T1; Core 1 O-Glycan T-Synthase; core 1 synthase, glycoprotein-N-acetylgalactosamine3-beta-galactosyltransferase, 1; Core 1 UDP-galactose:N-acetylgalactosamine-alpha-R beta1,3-galactosyltransferase 1; EC 2.4.1.122; glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1; T-synthase
• Gene ID: 56913
• mRNA Refseq: NM_020156
• Protein Refseq: NP_064541
• MIM: 610555
• UniProt ID: Q96QH4