Active Recombinant Human C1R, His-tagged

• Cat.No.: C1R-718H
• Product Overview: Recombinant Human C1R (Accession # NP_001724) Met1-Asp705, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Ser18 (A chain) & Ile464 (B chain)
• Form: Lyophilized from a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Gly-Arg-ThioBenzyl ester (Z-GR-SBzl), in the presence of 5,5"Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.The specific activity is >1,500 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human C1R, His-tagged has a calculated MW of 51 kDa (A chain) & 28 kDa (B chain). In SDS-PAGE migrates as 60 kDa & 40 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: C1R complement component 1, r subcomponent [ Homo sapiens ]
• Official Symbol: C1R
• Synonyms: C1R; complement component 1, r subcomponent; complement C1r subcomponent
• Gene ID: 715
• mRNA Refseq: NM_001733
• Protein Refseq: NP_001724
• MIM: 613785
• UniProt ID: P00736
• Chromosome Location: 12p13.31