Active Recombinant Human CAMKK1, GST-tagged

• Cat.No.: CAMKK1-1374H
• Product Overview: Recombinant full-length human CAMKK1 was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.

Specification

• Description: CAMKK1 or CAMKKα is a Ca(2+)/calmodulin-dependent protein kinase that activatesCaM-kinases I and IV via phosphorylation of their Thr(177) and Thr(196) residues, respectively. Recent studies have shown that the activity of CAMKK1 is decreased upon phosphorylation by cAMP-dependent protein kinase (PKA) The CAMKKalpha has been identified in intact cells as AMPKKs, predicting a significant role for this kinase in regulating AMPK activity in vivo. It has been shown that 2-deoxyglucose- and ionomycin-stimulated AMPK activity is substantially reduced in HeLa cells transfected with small interfering RNAs specific for CAMKKα.
• Source: Sf9 Cells
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 10 nmol/min/mg
• Molecular Mass: ~94 kDa
• Protein length: Full length
• Purity: >90%
• Applications: Kinase Assay, Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1 μg/μl

Gene Information

• Gene Name: CAMKK1 calcium/calmodulin-dependent protein kinase kinase 1, alpha [ Homo sapiens ]
• Official Symbol: CAMKK1
• Synonyms: CAMKK1; calcium/calmodulin-dependent protein kinase kinase 1, alpha; calcium/calmodulin-dependent protein kinase kinase 1; CAMKKA; DKFZp761M0423; MGC34095; caMKK 1; caM-KK 1; caM-KK alpha; CAMKK alpha protein; caM-kinase kinase 1; caM-kinase IV kinase; caM-kinase kinase alpha; calcium/calmodulin-dependent protein kinase kinase alpha
• Gene ID: 84254
• mRNA Refseq: NM_032294
• Protein Refseq: NP_115670
• MIM: 611411
• UniProt ID: Q8N5S9
• Chromosome Location: 17p13.3
• Pathway: Activation of NMDA receptor upon glutamate binding and postsynaptic events, organism-specific biosystem; Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; CREB phosphorylation through the activation of CaMKK, organism-specific biosystem; Neuronal System, organism-specific biosystem; Neurotransmitter Receptor Binding And Downstream Transmission In ThePostsynaptic Cell, organism-specific biosystem; Post NMDA receptor activation events, organism-specific biosystem
• Function: ATP binding; calmodulin binding; calmodulin-dependent protein kinase activity; nucleotide binding