Active Recombinant Human CASP8, His-tagged

• Cat.No.: CASP8-26H
• Product Overview: Recombinant Human CASP8 (a.a: 200 – 496) fussed with His tag at C-terminal was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 200-496 a.a.
• Description: Caspase-8 is a member of the caspase (cysteine aspartate protease) family of proteins, and has been shown to be an executioner protein of apoptosis. Caspase-8 has been associated with chronic inflammation and neurodegenerative disorders including Alzheimer’s, Huntington’s, and Parkinson’s. Procaspase-8 is autocleaved between the prodomain and large protease subunit (p18). The cleavage product, p30, contains both the large (p18) and small (p10 [His-tag]) protease subunits. p30 is further processed to the p18 and p10 subunits, which dimerize to form active enzyme.
• Form: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, 3 mM DTT and 20% glycerol.
• Bio-activity: ≥ 1145 pmol/min/µg
• Molecular Mass: 34.5 kDa
• Applications: Useful for the study of enzyme kinetics, screening inhibitors,and selectivity profiling.
• Stability: >6 months at -80 centigrade.
• Warning: Avoid freeze/thaw cycles.

Gene Information

• Gene Name: CASP8 caspase 8, apoptosis-related cysteine peptidase [ Homo sapiens ]
• Official Symbol: CASP8
• Synonyms: CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8; caspase-8; FADD-homologous ICE/CED-3-like protease; FADD-like ICE; ICE-like apoptotic protease 5; MACH-alpha-1/2/3 protein; MACH-beta-1/2/3/4 protein; MORT1-associated ced-3 homolog; apoptotic cysteine protease; apoptotic protease Mch-5; caspase 8, apoptosis-related cysteine protease
• Gene ID: 841
• mRNA Refseq: NM_001228
• Protein Refseq: NP_001219
• MIM: 601763
• UniProt ID: Q14790
• Chromosome Location: 2q33-q34
• Pathway: AGE/RAGE pathway, organism-specific biosystem; Activated TLR4 signalling, organism-specific biosystem; Alzheimer's disease, conserved biosystem
• Function: cysteine-type endopeptidase activity; cysteine-type endopeptidase activity involved in apoptotic process; death effector domain binding