Active Recombinant Human CCN1 protein, Fc-tagged

• Cat.No.: CCN1-1288H
• Product Overview: Recombinant Human Cyr61/CCN1 Protein is produced by Human Cell expression system. The target protein is expressed with sequence (Thr25-Asp381) of human Cyr61/CCN1 (Accession #O00622) fused with an Fc tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Thr25-Asp381
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion. Recombinant Human Cyr61/CCN1 Fc Chimera immobilized at 1.5 μg/mL (100 μL/well), will mediate >25% BALB/3T3 cell adhesion (3 x 104 cell/well added).
• Molecular Mass: 75 kDa
• Endotoxin: < 1 EU/μg of the protein by LAL method.
• Purity: > 95% by SDS-PAGE.
• Storage: Store the lyophilized protein at -20°C to -80 °C for long term.
  After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
• Reconstitution: Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.