Active Recombinant Human CD117 Protein, Fctagged

• Cat.No.: KIT-61H
• Product Overview: CD117 dimer protein contains a CD117 extracellular domain (UniProt# P10721) fused with a proprietary cis-dimer motif followed by a Fc tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293T
• Tag: Fc
• Protein Length: 25-524 aa
• Description: Human cluster of differentiation 117 (CD117), is a member of the Type III receptor tyrosine kinase family. CD117 is also known as KIT, C-Kit, mast/stem cell growth factor receptor (SCFR), KIT proto-oncogene receptor tyrosine kinase, and MASTC. CD117 contains an extracellular domain with five immunoglobulin-like loops, a transmembrane domain, a juxtamembrane domain, and an intracellular domain. CD117 is a Type I transmembrane protein, expressed on hematopoietic stem cells, mast cells, melanocytes, germ cells, and interstitial cells of Cajal. CD117 exists as a monomer under normal physiological conditions. Upon binding to its natural ligand, stem cell factor (SCF), homodimerization occurs between two CD117 monomers; this homodimerization is essential for its activation. However, oncogenic mutations can cause ligand-independent pathological dimerization and constitutive activation. CD117 is frequently overexpressed or dysregulated in cancers, including gastrointestinal stromal tumors, acute myeloid leukemia, melanoma, and small cell lung cancer. CD117 is a promising drug target, especially in precision oncology and regenerative medicine. Understanding CD117 dimerization and its activation is crucial for developing targeted therapeutics.
• Molecular Mass: 165 kDa
• Homodimer/Heterodimer: Homodimer
• Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition
• Application: Verified Applications: ELISA for CD117-specific antibody and stem cell factor (SCF) ligand protein binding assays. Suggested Applications: SPR & BLI for CD117-specific antibody and SCF protein binding assays. Animal immunization, RUO.
• Storage: At -80 centigrade
• Storage Buffer: 0.22μm filtered PBS, pH 7.4
• Shipping: Frozen Dry Ice

Gene Information

• Gene ID: 3815
• Gene Name: KIT v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog [Homo sapiens (human)]
• Official Symbol: 3815
• Synonyms: KIT; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; PBT, piebald trait; mast/stem cell growth factor receptor Kit; C Kit; CD117; SCFR; p145 c-kit; proto-oncogene c-Kit; piebald trait protein; soluble KIT variant 1; tyrosine-protein kinase Kit; proto-oncogene tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein; PBT; C-Kit
• mRNA Refseq: NM_000222
• Official Symbol 2: KIT
• Gene ID 2: 3815
• Gene Name 2: KIT v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog [Homo sapiens (human)]
• mRNA Refseq 2: NM_000222
• Protein Refseq 2: NP_000213
• MIM 2: 164920
• UniProt ID 2: P10721