Active Recombinant Human CD19 protein, His-tagged, R-PE labeled

• Cat.No.: CD19-3309HP
• Product Overview: Recombinant Human CD19 protein, His-tagged was Conjugated with NH2-Reactive R-PE(R-Phycoerythrin).

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met1-Lys291
• Form: Lyophilized from sterile 50 mM Tris, pH 8.0.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized Human CD19 His at 2 μg/ml (100 μl/well) can bind Anti-CD19 Antibody, the EC50 of Anti-CD19 Antibody is 5-40 ng/mL.
• Molecular Mass: The recombinant human CD19 consists of 283 amino acids and predictes a molecular mass of 31.6 kDa. In SDS-PAGE under reducing conditions, rhCD19 migrates as an approximately 47 kDa band due to glycosylation.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Conjugation: R-PE
• Publications:
  Transient blockade of TBK1/IKKε allows efficient transduction of primary human natural killer cells with vesicular stomatitis virus G-pseudotyped lentiviral vectors (2021)
  Cytokine and reactivity profiles in SLE patients following anti-CD19 CART therapy (2023)
  Homology-Directed Recombination for Enhanced Engineering of Chimeric Antigen Receptor T Cells (2017)
  Sensitive and adaptable pharmacological control of CAR T cells through extracellular receptor dimerization (2019)
  Development of a cGMP-compliant process to manufacture donor-derived, CD45RA-depleted memory CD19-CAR T cells (2022)

Gene Information

• Gene Name: CD19 CD19 molecule [ Homo sapiens ]
• Official Symbol: CD19
• Synonyms: CD19; CD19 molecule; CD19 antigen; B-lymphocyte antigen CD19; differentiation antigen CD19; T-cell surface antigen Leu-12; B-lymphocyte surface antigen B4; B4; CVID3; MGC12802
• Gene ID: 930
• mRNA Refseq: NM_001178098
• Protein Refseq: NP_001171569
• MIM: 107265
• UniProt ID: P15391