Active Recombinant Human CD244 protein, Fc-Avi-tagged, Biotinylated

• Cat.No.: CD244-2845H
• Product Overview: Biotinylated Recombinant Human CD244 protein(Leu 24-Gln 186), fused with Fc and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc-Avi
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH 7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human 4-1BB, Fc,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind UreluMab, Human IgG4 with a linear range of 0.1-6 ng/mL.Immobilized Human 4-1BB Ligand, Mouse IgG2a Fc Tag, low endotoxin at 2 μg/mL (100 μL/well) can bind Biotinylated Human 4-1BB, Fc,Avitag with a linear range of 0.2-3 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).The protein has a calculated MW of 45.4 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein Length: Leu 24-Gln 186
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CD244 CD244 molecule, natural killer cell receptor 2B4 [ Homo sapiens ]
• Official Symbol: CD244
• Synonyms: CD244; CD244 molecule, natural killer cell receptor 2B4; CD244 natural killer cell receptor 2B4 , natural killer cell receptor 2B4; natural killer cell receptor 2B4; 2B4; NAIL; NKR2B4; Nmrk; SLAMF4; h2B4; NK cell activation-inducing ligand; NK cell type I receptor protein 2B4; NK cell activation inducing ligand NAIL
• Gene ID: 51744
• mRNA Refseq: NM_001166663
• Protein Refseq: NP_001160135
• MIM: 605554
• UniProt ID: Q9BZW8