Active Recombinant Human CD3E and CE3D Heterodimer Protein, His&Avi tagged

• Cat.No.: CD3-18H
• Product Overview: CD3-epsilon/delta protein heterodimer contains a CD3-epsilon extracellular domain (UniProt# P07766, amino acids Asp23-Asp126) and a CD3-delta extracellular domain (UniProt# P04234, amino acids Phe22-Ala105) fused with a proprietary cis-dimer motif followed by a His-tag at the epsilon C-terminus and an Avi-tag at the delta C-terminus was expressed in HEK293T.

Specification

• Species: Human
• Source: HEK293T
• Tag: Avi&His
• Protein Length: CD3E: 23-126 aa CD3D: 22-105 aa
• Description: Human cluster of differentiation 3 (CD3) epsilon/delta (CD3δε, CD3-epsilon/delta) heterodimer contains CD3-epsilon and CD3-delta within the CD3 complex. CD3 complex is a crucial part of the T cell receptor (TCR). CD3-epsilon and CD3-delta are Type I transmembrane proteins and members of the immunoglobulin superfamily. CD3-epsilon and CD3-delta each contain an extracellular domain composed of an immunoglobulin domain followed by a short a membrane-proximal connecting peptide, a transmembrane domain, and an intracellular domain. CD3-epsilon/delta together with CD3-epsilon/gamma heterodimer, T-cell receptor-alpha/beta (TCR), and CD3-zeta homodimer make up the TCR complex. CD3 can act as a marker antigen due to its presence in almost all T-cell lymphomas and leukemias and can be used to distinguish them from other cancers. Because CD3 is required for T-cell activation, it has become an important target for developing cancer therapeutics, as well as immunosuppressant therapies for type 1 diabetes and other autoimmune diseases. In the T cell receptor, CD3-epsilon/delta is responsible for transmitting activation signals when the TCR recognizes an antigen and is involved in activating both the cytotoxic T cell and T helper cells. Therefore, a recombinant CD3-epsilon/delta protein heterodimer mimicking the CD3-epsilon/delta heterodimer native conformation can be critical for target for drug discovery and immunotherapy research.
• Molecular Mass: 38 kDa
• Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition
• Application: Verified Applications: ELISA for CD3-epsilon-specific and CD3-delta-specific antibody binding assays. Suggested Applications: SPR & BLI for CD3-epsilon-specific antibodies and CD3-delta-specific antibodies. Animal immunization, RUO.
• Storage: Store at -80 centigrade.
• Storage Buffer: 0.22μm filtered PBS, pH 7.4
• Shipping: Frozen Dry Ice

Gene Information

• Gene Name: CD3E CD3 epsilon subunit of T-cell receptor complex [ Homo sapiens (human) ]
• Official Symbol: CD3E
• Synonyms: CD3E; CD3 epsilon subunit of T-cell receptor complex; T3E; TCRE; IMD18; CD3epsilon; T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain
• Gene ID: 916
• mRNA Refseq: NM_000733
• Protein Refseq: NP_000724
• MIM: 186830
• UniProt ID: P07766
• Gene Name 2: CD3D CD3 delta subunit of T-cell receptor complex [ Homo sapiens (human) ]
• Official Symbol 2: CD3D
• Synonyms 2: CD3D; CD3 delta subunit of T-cell receptor complex; T3D; IMD19; CD3DELTA; CD3-DELTA; T-cell surface glycoprotein CD3 delta chain; CD3 antigen, delta subunit; CD3 delta; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); OKT3, delta chain; T-cell receptor T3 delta chain
• Gene ID 2: 915
• mRNA Refseq 2: NM_000732
• Protein Refseq 2: NP_000723
• MIM 2: 186790
• UniProt ID 2: P04234