| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
Avi&Fc&Flag&His |
| Protein Length : |
Asp 23-Asp 126 (CD3E) & Phe 22-Ala 105 (CD3D) |
| Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
| Bio-activity : |
Immobilized Biotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His,Avitag&Fc,Flag,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3) with a linear range of 0.4-3 ng/mL.Immobilized Biotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His,Avitag&Fc,Flag,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind UCHT1 with a linear range of 0.1-2 ng/mL. |
| Molecular Mass : |
Biotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His,Avitag&Fc,Flag,Avitag is produced by co-expression of CD3E and CD3D, has a calculated MW of 45.1 kDa (CD3E) & 42.6 kDa (CD3D). Subunit CD3E is fused with a human IgG1 Fc fragment at the C-terminus, followed by an Avi tag (Avitag™), the polyhistidine tag is inserted in-between the Fc and Avi tags at the C-terminus and subunit CD3D contains a human IgG1 Fc fragment at the C-terminus, followed by an Avi tag (Avitag™), the flag tag is inserted in-between the Fc and Avi tags at the C-terminus. As a result of glycosylation, the heterodimer protein migrates as 50-60 kDa under reducing (R) condition. |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>90% as determined by SDS-PAGE.>90% as determined by SEC-MALS. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
