Active Recombinant Human CD3E & CD3D protein, His-tagged

• Cat.No.: CD3E & CD3D-2959H
• Product Overview: Recombinant Human CD3E&CD3D protein(Asp 23-Asp 126 (CD3E) & Phe 22-Ala 105 (CD3D)), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), premium grade at 1 μg/mL (100 μL/well) can bind HRP-Human CD3E & CD3D Heterodimer Protein, His Tag&Tag Free with a linear range of 0.1-8 ng/mL.Immobilized UCHT1 at 1 μg/mL (100 μL/well) can bind HRP-Human CD3E & CD3D Heterodimer Protein, His Tag&Tag Free with a linear range of 0.1-2 ng/mL.Immobilized Mouse Anti-Human CD3 (SP34-2) at 1 μg/mL (100 μL/well) can bind HRP-Human CD3E & CD3D Heterodimer Protein, His Tag&Tag Free with a linear range of 0.1-4 ng/mL.Immobilized Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), premium grade at 1 μg/mL (100 μL/well) can bind HRP-Human CD3E & CD3D Heterodimer Protein, His Tag&Tag Free with a linear range of 0.1-6 ng/mL.Immobilized Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), premium grade at 1 μg/mL (100 μL/well) can bind HRP-Human CD3E & CD3D Heterodimer Protein, His Tag&Tag Free with a linear range of 0.1-6 ng/mL.
• Molecular Mass: HRP-Human CD3E & CD3D Heterodimer Protein, His Tag&Tag Free is produced by co-expression of CD3E and CD3D, has a calculated MW of 16.6 kDa (CD3E) and 13.4 kDa (CD3D). Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3D contains no tag.
• Protein Length: Asp 23-Asp 126 (CD3E) & Phe 22-Ala 105 (CD3D)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CD3E CD3e molecule, epsilon (CD3-TCR complex) [ Homo sapiens ]
• Official Symbol: CD3E
• Synonyms: CD3E; CD3e molecule, epsilon (CD3-TCR complex); CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell antigen receptor complex, epsilon subunit of T3; T3E; TCRE; FLJ18683
• Gene ID: 916
• mRNA Refseq: NM_000733
• Protein Refseq: NP_000724
• UniProt ID: P07766

Reviews

03/22/2023

Manufacturers with the capacity for bulk production can offer CD3E protein in larger quantities, which is advantageous for researchers working on large-scale experiments or collaborations.

09/12/2021

bulk production often leads to economies of scale, making the CD3E protein more cost-effective for research budgets.

06/17/2018

Manufacturers can actively seek collaborations and partnerships with researchers to develop new applications or optimize methodologies using CD3E protein.

Q&As

Can CD3E be used to assess the effectiveness of cancer immunotherapies?

Yes, CD3E levels and T-cell function can be monitored to evaluate the response to cancer immunotherapies like checkpoint inhibitors.

Are there any CD3E-targeted therapies?

There are currently no approved therapies that directly target CD3E, but it is a target for research in developing immunotherapies and cancer treatments.

Are there any ongoing clinical trials involving CD3E?

There may be ongoing clinical trials using CD3E-targeted therapies in the context of cancer and autoimmune diseases. It's important to check clinical trial databases for specific studies.

Can CD3E levels be used to monitor the progression of HIV?

Yes, monitoring CD3E levels in HIV-infected individuals can provide valuable information about the progression of the disease and the effectiveness of antiretroviral therapy.

How is CD3E used in allergy diagnostics?

CD3E can be used in research to study T-cell responses in allergic conditions, helping to better understand and diagnose allergies.