Active Recombinant Human CD52 Protein, Fc-Avi-tagged, Biotinylated

• Cat.No.: CD52-390H
• Product Overview: Biotinylated Human CD52, Fc Tag is expressed from HEK293 cells. It contains AA Gly 25 - Ser 36 (Accession # P31358-1 ). This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&Fc
• Protein Length: 25-36 a.a.
• Predicted N Terminal: Gly 25
• Form: Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
• Bio-activity: Immobilized Anti CD52 MAb, Human IgG1 at 10 ug/mL (100 uL/well) can bind Biotinylated Human CD52, Fc Tag with a linear range of 0.625-10 ug/mL.
• Molecular Mass: The protein has a calculated MW of 29.7 kDa. As a result of glycosylation, the protein migrates as 38-45 kDa under reducing condition, and 66-100 kDa under non-reducing condition (SDS-PAGE).
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Conjugation: Biotin

Gene Information

• Gene Name: CD52 CD52 molecule [ Homo sapiens ]
• Official Symbol: CD52
• Synonyms: CD52; CD52 molecule; CD52 antigen (CAMPATH 1 antigen) , CDW52; CAMPATH-1 antigen; he5; cambridge pathology 1 antigen; epididymal secretory protein E5; CD52 antigen (CAMPATH-1 antigen); CDW52 antigen (CAMPATH-1 antigen); human epididymis-specific protein 5; CDW52
• Gene ID: 1043
• mRNA Refseq: NM_001803
• Protein Refseq: NP_001794
• MIM: 114280
• UniProt ID: P31358