Active Recombinant Human CD58 protein, hFc-tagged
| Cat.No. : | CD58-543H |
| Product Overview : | Recombinant Human CD58 protein(Q9BRW0)(Met1-Arg215), fused with hFc tag, was expressed in HEK293. |
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| Species : | Human |
| Source : | HEK293 |
| Tag : | Fc |
| Protein Length : | Met1-Arg215 |
| Form : | Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
| Bio-activity : | 1. Measured by its binding ability in a functional ELISA. Immobilized human CD2-His (Cat:10982-H08H) at 10 μg/ml (100 μl/well) can bind human CD58-Fc, The EC50 of human CD58-Fc is 0.04-0.1 μg/ml.2. Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus CD2-His (Cat:90300-C08H) at 10 μg/ml (100 μl/well) can bind human CD58-Fc, The EC50 of human CD58-Fc is 0.04-0.10 μg/ml. |
| Molecular Mass : | The recombinant human CD58/Fc is a disulfide-linked homodimer. The reduced monomer comprises 428 amino acids and has a predicted molecular mass of 48.5 kDa. The apparent molecular mass of the protein is approximately 68 kDa in SDS-PAGE under reducing conditions. |
| Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
| Purity : | > 95 % as determined by SDS-PAGE |
| Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| Gene Name | CD58 CD58 molecule [ Homo sapiens ] |
| Official Symbol | CD58 |
| Synonyms | CD58; CD58 molecule; CD58 antigen, (lymphocyte function associated antigen 3) , LFA3; lymphocyte function-associated antigen 3; surface glycoprotein LFA-3; CD58 antigen, (lymphocyte function-associated antigen 3); ag3; LFA3; LFA-3; FLJ23181; FLJ43722; |
| Gene ID | 965 |
| mRNA Refseq | NM_001144822 |
| Protein Refseq | NP_001138294 |
| MIM | 153420 |
| UniProt ID | P19256 |
Not For Human Consumption!
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