Active Recombinant Human CD96 protein, His-tagged, Biotinylated

• Cat.No.: CD96-2181H
• Product Overview: Biotinylated Recombinant Human CD96 protein(P40200-2)(Met1-Met503), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met1-Met503
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Immobilized human CD155/PVR (Fc Tag)（10109-H02H）at 2 μg/mL (100 μL/well) can bind CD96 (His Tag), Biotinylated, the EC50 of CD96 (His Tag), Biotinylated is 150-800 ng/mL.
• Molecular Mass: The recombinant human CD96 consists of 493 amino acids and predictes a molecular mass of 55 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh CD96 is approximately 120-130 kDa due to high glycosylation.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Conjugation: Biotin

Gene Information

• Gene Name: CD96 CD96 molecule [ Homo sapiens ]
• Official Symbol: CD96
• Synonyms: CD96; CD96 molecule; CD96 antigen; T-cell surface protein tactile; TACTILE; cell surface antigen CD96; T cell activation, increased late expression; t cell-activated increased late expression protein; MGC22596; DKFZp667E2122
• Gene ID: 10225
• mRNA Refseq: NM_005816
• Protein Refseq: NP_005807
• MIM: 606037
• UniProt ID: P40200