Active Recombinant Human CDK3/CCNE2 protein, GST-tagged

• Cat.No.: CDK3-CCNE2-312H
• Product Overview: Recombinant full-length human CDK3 and CCNE2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Description: CDK3/CyclinE is a member of the cyclin-dependent protein kinase family and promotes entry into S phase, in part by activating members of the E2F family of transcription factors. CDK3 can also associate with cyclin C and phosphorylates the retinoblastoma 1 protein to promote exit from G0. The CDK3 gene has been mapped to chromosomal location of 17q22-qter, telomeric to the BRCA1 gene by somatic cell hybrids analysis. The presence of a single point mutation in the CDK3 gene from several Mus musculus strains commonly used in the laboratory has been reported.
• Form: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol
• Bio-activity: The specific activity of CDK3/CyclinE2 was determined to be 80nmol/min/mg as per activity protocol.
• Molecular Mass: CDK3 ~60 kDa and CCNE2 ~72 kDa
• Purity: The purity of CDK3/CCNE2 was determined to be >95% by densitometry.
• Applications: Kinase Assay; WB
• Stability: 1 year at -70 centigrade
• Storage: Store product at -70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μL

Gene Information

• Gene Name: cyclin dependent kinase 3 [ Homo sapiens (human) ]; cyclin E2 [ Homo sapiens (human) ]
• Official Symbol: CDK3; CCNE2
• Synonyms: CDK3; CCNE2; CYCE2
• Gene ID: 1018; 9134
• mRNA Refseq: NM_001258; NM_057749
• Protein Refseq: NP_001249; NP_001228
• MIM: 123828; 603775
• UniProt ID: Q00526; P24864