Active Recombinant Human CFHR4 Protein, His-tagged

• Cat.No.: CFHR4-83H
• Product Overview: Recombinant human Complement Factor H-related 4/CFHR4 protein (Gln19-Glu331), with a C-terminal 10-His tag, expressed in Spodoptera frugiperda, Sf 21 (baculovirus).

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 19-331
• Description: This gene is a member of the complement factor H (CFH) gene family, and encodes one of the 5 CFH-related (CFHR) proteins. These 5 genes are closely linked to the CFH gene on chromosome 1q31-q32. The CFHRs are secreted plasma proteins synthesized primarily by the hepatocytes, and composed of highly-related short consensus repeats (SCRs). This protein enhances the cofactor activity of CFH, and is involved in complement regulation. It can associate with lipoproteins and may play a role in lipid metabolism. Alternatively spliced transcript variants encoding different isoforms (varying in the number of SCRs) have been described for this gene.
• Bio-activity: Measured by its binding ability in a functional ELISA. When Recombinant Mouse Complement Component C3d is immobilized at 2 μg/mL (100 μL/well), Recombinant Human Complement Factor H‑related 4/CFHR4 binds with an ED50 of 1-6 ng/mL.
• Molecular Mass: 37 kDa
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Applications: Bioactivity
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, ≤ -20 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with Trehalose.
• Reconstitution: Reconstitute at 500 μg/mL in water.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: CFHR4 complement factor H related 4 [ Homo sapiens (human) ]
• Official Symbol: CFHR4
• Synonyms: CFHR4; complement factor H related 4; FHR4; CFHL4; FHR-4; complement factor H-related protein 4
• Gene ID: 10877
• mRNA Refseq: NM_006684
• Protein Refseq: NP_006675
• MIM: 605337
• UniProt ID: Q92496