Active Recombinant Human Chemokine (C-X-C motif) Ligand 17

• Cat.No.: CXCL17-436H
• Product Overview: Recombinant Human CXCL17is expressed in E. coli, which has a molecule weight of 11.3 kDa. Leu24­ Leu119.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Dendritic cell and monocytechemokine­like protein (DMC), also known as VEGF­correlated chemokine­1 (VCC­1)and CXCL17, is a novel secreted molecule that belongs to the intercrine alpha(or CXC) family of chemokines. It has no predicted N­glycosylation sites, so cleavageof a 22 amino acid (aa) signal sequence likely results in a mature human DMC of97 aa and 11 kDa. DMC expression is increased in endothelial cells when they areinduced to form tubes in vitro. Transgenic overexpression in NIH3T3 cells causesup­regulation of proteins such as VEGF and FGF basic, and increases cell growthrate and tumorigenicity. DMC and two other chemokines that play roles in angiogenesis,CXCL1/GRO and CXCL8/IL­8, show significantly correlated expression with that ofVEGF in primary lung, breast and esophageal tumors. DMC is therefore suggestedto play a role in tumor angiogenesis. Mature human DMC shares 73%, 71% and 64%amino acid sequence identity with bovine, mouse and rat DMC, respectively.
• Form: Lyophilized from a 0.2μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• N-terminal Sequence: Leu24
• Molecular Weight: 11.3 kDa
• Activity: Measured by its abilityto induce VEGF expression in mouse endothelial cells. The ED50 for this effectis typically 1 ­ 5 µg/mL.
• Purity: >95%, by SDS­PAGEunder reducing conditions and visualized by silver stain.
• Endotoxin Level: <1.0 EU per 1 μg ofthe protein by the LAL method.
• Reconstitution: Reconstitute at 100μg/mL in PBS that containing at least 0.1% human or bovine serum albumin.
• Storage: Use a manual defrostfreezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.
• OfficialSymbol: CXCL17

Gene Information

• Gene Name: CXCL17 chemokine (C-X-C motif)ligand 17 [ Homo sapiens ]
• Synonyms: CXCL17; chemokine (C-X-Cmotif) ligand 17; DMC; VCC1; Dcip1; VCC-1; UNQ473; MGC138300; VEGF co-regulatedchemokine 1; C-X-C motif chemokine 17; dendritic cell and monocyte chemokine-likeprotein
• Gene ID: 284340
• mRNA Refseq: NM_198477
• Protein Refseq: NP_940879
• MIM: 611387
• UniProt ID: Q6UXB2
• Chromosome Location: 19q13.2