Active Recombinant Human CPA1 protein, His-tagged

• Cat.No.: CPA1-3176H
• Product Overview: Recombinant Human CPA1 protein(NP_001859.1)(Met1-Tyr419), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met1-Tyr419
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to cleave the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of 5,5'Dithiobis (2-nitrobenzoic acid) (DTNB). The specific activity is >3,500 pmoles/min/μg.
• Molecular Mass: The secreted recombinant human CPA1 (pro form) consists of 414 amino acids and has a predicted molecular mass of 47 kDa. In SDS-PAGE under reducing conditions, it migrates with the apparent molecular mass of 43 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CPA1 carboxypeptidase A1 (pancreatic) [ Homo sapiens ]
• Official Symbol: CPA1
• Synonyms: CPA1; carboxypeptidase A1 (pancreatic); CPA; carboxypeptidase A1; pancreatic carboxypeptidase A
• Gene ID: 1357
• mRNA Refseq: NM_001868
• Protein Refseq: NP_001859
• MIM: 114850
• UniProt ID: P15085