Active Recombinant Human CPE protein, His-tagged
| Cat.No. : | CPE-659H |
| Product Overview : | Recombinant Human CPE protein(NP_001864.1)(Met1-Ser453), fused with His tag, was expressed in HEK293. |
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| Source : | HEK293 |
| Species : | Human |
| Tag : | His |
| Form : | Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
| Bio-activity : | Measured by its ability to cleave a peptide substrate, benzoyl-AR-OH. The product, Arg, reacted with orthophthaldialdehyde (OPA) to form a fluorescent molecule. The specific activity is >12,000 pmoles/min/μg. |
| Molecular Mass : | The recombinant human CPE comprises 439 amino acids and predicts a molecular mass of 49.4 kDa. As a result of glycosylation, the apparent molecular mass of rhCPE is approximately 53 kDa in SDS-PAGE under reducing conditions. |
| Protein Length : | Met1-Ser453 |
| Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
| Purity : | > 90 % as determined by SDS-PAGE |
| Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| Gene Name : | CPE carboxypeptidase E [ Homo sapiens ] |
| Official Symbol : | CPE |
| Synonyms : | CPE; carboxypeptidase E; carboxypeptidase H; cobalt stimulated chromaffin granule carboxypeptidase; enkephalin convertase; insulin granule associated carboxypeptidase; CPH; prohormone-processing carboxypeptidase; insulin granule-associated carboxypeptidase; cobalt-stimulated chromaffin granule carboxypeptidase; |
| Gene ID : | 1363 |
| mRNA Refseq : | NM_001873 |
| Protein Refseq : | NP_001864 |
| MIM : | 114855 |
| UniProt ID : | P16870 |
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| ◆ Native Protein | ||
| cpe-001C | Active Native C. perfringens Enterotoxin | +Inquiry |
| ◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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