Active Recombinant Human CSF3 protein(Ala30-Pro204), hFc-tagged
Cat.No. : | CSF3-203H |
Product Overview : | Recombinant Human GCSF isoform b (NP_757373.1) (Ala 30-Pro 204) was expressed in HEK293, fused with the Fc region of Human IgG1 at the N-terminus. |
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Source : | HEK293 |
Species : | Human |
Tag : | N-hFc |
Protein length : | Ala30-Pro204 |
Form : | Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | Measured in a cell prolifeRation assay using a murine myeloblastic cell line, NFS-60. The ED50 for this effect is typically 0.2-0.8 ng/ml. |
Molecular Mass : | The recombinant mature human GCSFb/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 412 amino acids and has a calculated molecular mass of 45.4 kDa. As a result of glycosylation, the rh GCSFb/Fc monomer migRates as an approximately 48 kDa protein in SDS-PAGE under reducing conditions. |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 97 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | CSF3 colony stimulating factor 3 (granulocyte) [ Homo sapiens ] |
Official Symbol : | CSF3 |
Synonyms : | CSF3; colony stimulating factor 3 (granulocyte); C17orf33, chromosome 17 open reading frame 33 , G CSF, GCSF; granulocyte colony-stimulating factor; filgrastim; granulocyte colony stimulating factor; lenograstim; MGC45931; pluripoietin; GCSF; CSF3OS; C17orf33; |
Gene ID : | 1440 |
mRNA Refseq : | NM_000759 |
Protein Refseq : | NP_000750 |
MIM : | 138970 |
UniProt ID : | P09919 |
Products Types
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CSF3-1163H | Recombinant Human CSF3 protein(Met1-Pro204) | +Inquiry |
CSF3-4407C | Recombinant Chicken CSF3 Protein | +Inquiry |
CSF3-30H | Active Recombinant Human CSF3 Protein (Thr31-Pro204), N-His tagged, Animal-free, Carrier-free | +Inquiry |
◆ Lysates | ||
CSF3-2948HCL | Recombinant Human CSF3 cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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