Active Recombinant Human CSPG5, His-tagged

• Cat.No.: CSPG5-416H
• Product Overview: Recombinant Human CSPG5 (Accession # AAQ04774) Val31-Gln420, fused with a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Protein Length: 31-420 a.a.
• Predicted N Terminal: Val31
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its ability to inhibit Recombinant Human Midkine induced neurite outgrowth in E16-E18 rat embryonic cortical neurons.When 5 x 10^4 neurons per well are added to a plate pre-incubated with serial dilutions of rhNeuroglycan-C and 1 µg/mL of rhMidkine, the neurite outgrowth promotion is significantly inhibited in a dose dependent manner with an effect typically at 0.6 - 1.2 µg/mL.
• Molecular Mass: Recombinant Human CSPG5, His-tagged has a calculated MW of 41.9 kDa. In SDS-PAGE migrates as 80-100 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: CSPG5 chondroitin sulfate proteoglycan 5 (neuroglycan C) [ Homo sapiens ]
• Official Symbol: CSPG5
• Synonyms: CSPG5; chondroitin sulfate proteoglycan 5 (neuroglycan C); chondroitin sulfate proteoglycan 5; NGC; acidic leucine-rich EGF-like domain-containing brain protein; MGC44034
• Gene ID: 10675
• mRNA Refseq: NM_001206942
• Protein Refseq: NP_001193871
• MIM: 606775
• UniProt ID: O95196
• Chromosome Location: 3p21.3