Active Recombinant Human CUL2/RBX1 protein
Cat.No. : | CUL2-133H |
Product Overview : | Recombinant Human CUL2/RBX1 was coexpressed in Insect cells. |
- Specification
- Gene Information
- Related Products
Source : | Insect cells |
Species : | Human |
Form : | 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol |
Bio-activity : | E3 Ligase Assay: The activity of Cul2/Rbx1 was validated indirectly through its ability to act as a substrate for Neddylation in the presence of the NEDD8 E3 ligase His-DCNL2 and thioester-loaded His-UBE2M-NEDD8. Incubation of Cul2/Rbx1 and thioester loaded His-UBE2M-NEDD8 in the presence or absence of His-DCNL2 at 4˚C was compared at two time points T0 and T2 minutes. Neddylation of the Cul2 subunit in the presence of His-DCNL2 was demonstrated. |
Molecular Mass : | Cul2=~87.1kDa; Rbx1=~12.3kDa |
Purity : | >75% by InstantBlue™ SDS-PAGE |
Stability : | 12 months at -70 centigrade; aliquot as required |
Storage : | Store product at –70 centigrade |
Concentration : | 0.5mg/ml |
Gene Name : | CUL2 cullin 2 [ Homo sapiens ] |
Official Symbol : | CUL2 |
Synonyms : | CUL2; cullin 2; cullin-2; CUL-2; MGC131970; |
Gene ID : | 8453 |
mRNA Refseq : | NM_001198777 |
Protein Refseq : | NP_001185706 |
MIM : | 603135 |
UniProt ID : | Q13617 |
Chromosome Location : | 10p11.2 |
Pathway : | Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; ECV complex, organism-specific biosystem; Hypoxic and oxygen homeostasis regulation of HIF-1-alpha, organism-specific biosystem; |
Function : | protein binding; ubiquitin protein ligase binding; |
Products Types
◆ Recombinant Protein | ||
Cul2-2375M | Recombinant Mouse Cul2 Protein, Myc/DDK-tagged | +Inquiry |
CUL2-689H | Recombinant Human CUL2 Protein, His (Fc)-Avi-tagged | +Inquiry |
CUL2-1295H | Recombinant Human CUL2 Protein, His&GST-tagged | +Inquiry |
CUL2-2081M | Recombinant Mouse CUL2 Protein, His (Fc)-Avi-tagged | +Inquiry |
CUL2-921R | Recombinant Rhesus Macaque CUL2 Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
CUL2-7184HCL | Recombinant Human CUL2 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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