Active Recombinant Human DAG1 Protein (Gln28-Val749), C-Fc-tagged

• Cat.No.: DAG1-09H
• Product Overview: Recombinant Human Dystroglycan (Gln28-Val749) protein with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Human embryonic kidney cell.

Specification

• Description: This gene encodes dystroglycan, a central component of dystrophin-glycoprotein complex that links the extracellular matrix and the cytoskeleton in the skeletal muscle. The encoded preproprotein undergoes O- and N-glycosylation, and proteolytic processing to generate alpha and beta subunits. Certain mutations in this gene are known to cause distinct forms of muscular dystrophy. Alternative splicing results in multiple transcript variants, all encoding the same protein.
• Source: HEK293
• Species: Human
• Tag: C-Fc
• Form: Disulfide-linked homodimer
• Bio-activity: Measured by the ability of the immobilized protein to enhance the adhesion of H4 human neuroglioma cells. The ED50 for this effect is 1.5-9 μg/mL.
• Molecular Mass: Predicted Molecular Mass: 68 kDa (alpha subunit) & 37 kDa (beta subunit)
  SDS-PAGE: 70-100 kDa and 120-135 kDa (alpha subunit) & 45-55 kDa (beta subunit)
• Protein Length: Gln28-Val749
• N-terminal Sequence Analysis: Gln28 inferred from AA sequence (alpha subunit) & Ser654 (beta subunit)
• Endotoxin: < 0.10/μg of the protein by the LAL method.
• Purity: > 95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  2 weeks, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: DAG1 dystroglycan 1 [ Homo sapiens (human) ]
• Official Symbol: DAG1
• Synonyms: DAG1; dystroglycan 1; A3a; DAG; AGRNR; 156DAG; MDDGA9; MDDGC7; MDDGC9; LGMDR16; dystroglycan 1; dystroglycan 1 (dystrophin-associated glycoprotein 1)
• Gene ID: 1605
• mRNA Refseq: NM_004393
• Protein Refseq: NP_004384
• MIM: 128239
• UniProt ID: Q14118