Active Recombinant Human DLL3 protein, His-tagged, Low Endotoxin

• Cat.No.: DLL3-4233H
• Product Overview: Recombinant Human DLL3 protein(Q9NYJ7-1)(Val311-Arg427), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 311-427 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human DLL3 Domain (311-427), His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 13.54 kDa. Due to glycosylation, the protein migrates to 15-20 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: DLL3 delta-like 3 (Drosophila) [ Homo sapiens ]
• Official Symbol: DLL3
• Synonyms: DLL3; delta-like 3 (Drosophila); delta (Drosophila) like 3; delta-like protein 3; SCDO1; delta3; drosophila Delta homolog 3
• Gene ID: 10683
• mRNA Refseq: NM_016941
• Protein Refseq: NP_058637
• MIM: 602768
• UniProt ID: Q9NYJ7